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Journal: Cell reports

Article Title: Distinct subpopulations of ventral pallidal cholinergic projection neurons encode valence of olfactory stimuli

doi: 10.1016/j.celrep.2024.114009

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: A Hamilton syringe was slowly lowered to the VP and mice were injected with ~0.3 μL of the following viral vectors dependent on experiment: AAV 9 .DIO.TRE.hM4Di.P2A.m.Cherry (ADCD; packaged at UNC viral vector core), AAV 9 .RAM.d2tTA.TRE.mCherry.NLS-FLAG (RAM; Addgene # 63931, packaged at UNC viral vector core), AAV 9 .Syn.Flex-GCaMP6f.WPRE.SV40 (GCaMP6f; Addgene # 100837), AAV 8 .hSyn.DIO.hM4Di (Gi).mCherry (hM4Di; Addgene # 44362), AAV 9 .eSyn.eGFP (Vector Biolabs # VB4870), AAV 9 .RAM.d2tTA.TRE.Flex.tdTomato (FLEX-RAM; Addgene # 84468, packaged at NINDS Viral Vector Core).

Techniques: Virus, Plasmid Preparation, Recombinant, Software